Skip to main content
. 2016 Oct 27;205(1):113–124. doi: 10.1534/genetics.116.194027

Figure 3.

Figure 3

The sensitivity of H4-R36A cells to CENP-ACse4 overexpression is not due to H3 depletion. (A) Fivefold serial dilutions of the indicated strains [pGAL-3Flag-CSE4 (SBY10025), H4-R36A pGAL-3Flag-CSE4 (SBY9397), H3-V46A (SBY10392), H3-V46A pGAL-3Flag-CSE4 (SBY10511), H3-R49A (SBY10393), H3-R39A pGAL-3Flag-CSE4 (SBY10512), H4-I46A (SBY10394), and H4-I46A pGAL-3Flag-CSE4 (SBY10513)] were plated on glucose or galactose media at 23°. (B) Fivefold dilutions of the indicated strains [HHT2-HHF2 (SBY9401), hht2-hhf2∆ (SBY10730), pGAL-3Flag-CSE4 HHT2-HHF2 (SBY10025), pGAL-3Flag-CSE4 hht2-hhf2∆ (SBY10734), H4-R36A HHT2-HHF2 (SBY9365), H4-R36A hht2-hhf2∆ (SBY10778), H4-R36A pGAL-3Flag-CSE4 HHT2-HHF2 (SBY9397), and H4-R36A pGAL-3Flag-CSE4 hht2-hhf2∆ (SBY10782)] were plated on glucose or galactose media at 23°.