TABLE 1.
Primer and probe sequences for expression of mRNAs for SP-A, SP-D, SBD1, and HBD6
| Primer or probe specificity and primer or probe | Sequencea |
|---|---|
| SP-A | |
| Forward | 5′-TGACCCTTATGCTCCTCTGGAT-3′ |
| Reverse | 5′-GGGCTTCCAAGACAAACTTCCT-3′ |
| Probe | 5′-6FAM-TGGCTTCTGGCCTCGAGTGCG-TAMRA-3′ |
| SP-D | |
| Forward | 5′-ACGTTCTGCAGCTGAGAAT-3′ |
| Reverse | 5′-TCGGTCATGCTCAGGAAAGC-3′ |
| Probe | 5′-6FAM-TTGACTCAGCTGGCCACAGCCCAGAACA-TAMRA-3′ |
| SBD1 | |
| Forward | 5′-CCATAGGAATAAAGGCGTCTGTGT-3′ |
| Reverse | 5′-CGCGACAGGTGCCAATCT-3′ |
| Probe | 5′-6FAM-CCGAGCAGGTGCCCTAGACACATGA-TAMRA-3′ |
| HBD6 | |
| Forward | 5′-CCC CAG CCA AGA ATG CAT-3′ |
| Reverse | 5′-TCA TTT TTC CCG CAA TTG TTC-3′ |
| Probe | 5′-6FAM-TTTTGATGAGAAATGCAACAAACTTAAAGGGACA-TAMRA-3′ |
a
6FAM, 6-carboxyfluorescein (fluorescent reporter dye); TAMRA, 6-carboxytetramethylrhodamine (fluorescent quencher dye).