Table 2. Mutations in the constitutive mutants as compared to the parental strain JN29.2.
| Strain | Total number of SNPs and indels | SNPs or indels in coding region | Mutation in NRRL3_08194 | Position of mutation relative to ATG of NRRL3_08194 | Mutation in codon (bold) | Amino acid change | Predicted protein length (full length protein is 697 amino acids) |
|---|---|---|---|---|---|---|---|
| JN29.2_UV1 | 40 | 19 | G-T | 1372 | GAG-TAG | E to Stop | 457 aa |
| JN29.2_UV8 | 21 | 4 | G-A | 1577 | GGA-GAA | G to E (526) | 697 aa |
| JN29.2_S1 | 68 | 34 | Extra G | 1958 | GTT-GGT | V to G out of frame | 663 aa |
| JN29.2_S5 | 48 | 11 | C-T | 1105 | CAA-TAA | Q to Stop | 368 aa |
| JN29.2_S7 | 11 | 1 | T-C | 2027 | CTG-CCG | L-P (676) | 697 aa |