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. 2016 Oct 24;205(1):221–237. doi: 10.1534/genetics.116.195917

Figure 8.

Figure 8

Variants of murine EXOSC3, corresponding to PCH1b-associated EXOSC3 variants, are expressed at reduced level in a mouse neuronal cell line. The steady-state levels of murine Myc-EXOSC3-G31A or Myc-EXOSC3-W237R variant are decreased relative to wild-type Myc-EXOSC3 in mouse N2a cells. Lysates of mouse N2a cells transfected with bicistronic vectors expressing murine Myc-EXOSC3 or Myc-EXOSC3 variant (G31A; W237R) and GFP were analyzed by immunoblotting with anti-Myc antibody to detect Myc-EXOSC3 proteins, and anti-GFP antibody to detect GFP as a transfection control. eIF5A protein was detected with anti-eIF5A antibody as a loading control. Percentage of Myc-EXOSC3 protein relative to GFP and wild-type Myc-EXOSC3 (% Myc-Exosc3 Rel WT) is shown below each lane, and was calculated as described in Materials and Methods. Quantitation is for the specific experiment shown, but is representative of multiple experiments.