Table 2. lin-28 and hbl-1 are genetically downstream of the 3let-7s.
| Percentage of animals with alae formation | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Average seam cell numbera,b | Precocious alaea,d,g | Adult alaec,d | |||||||
| Line | Strain | Genotype/treatmente,f | L3 | L4 | No alae | Gapped | Complete | N | |
| 1 | RG733 | WT | 16(6) | 16(16) | 0(6) | 0 | 0 | 100 | 12 |
| 2 | ME357 | lin-4(e912); mir-48 mir-241(nDf51); lin-14(n179ts) mir-84(n4037) | — | 110.5(30) | — | 100 | 0 | 0 | 30 |
| 3 | RG733 | lin-28(RNAi) | 11.5(12) | 11.5(30) | 100(19) | — | — | — | — |
| 4 | ME357 | lin-4(e912); mir-48 mir-241(nDf51); lin-14(n179ts) mir-84(n4037); lin-28(RNAi) | 11(12)h | 11.3(20)h | 100(7)h | — | — | — | — |
| 5 | RG733 | hbl-1(RNAi) | 13.6(61) | 15.8(59) | 98(51) | — | — | — | — |
| 6 | ME357 | lin-4(e912); mir-48 mir-241(nDf51); lin-14(n179ts) mir-84(n4037); hbl-1(RNAi) | 18.4(45)i | 28.4(78)i,j | 2 (61)k | 41.2j | 41.2j | 17.6j | 68 |
a
Number of animals (n) scored for each genotype is indicated in parenthesis.
b
P-values determined using Student’s t-test.
c
Animals were scored at the end of the L4 stage for both seam cells and alae formation.
d
P-values determined using Fisher’s exact test.
e
All strains contain wIs78 (ajm-1::GFP, SCMp::GFP) to mark seam cells.
f
Strains were maintained at 20°.
g
Animals were scored at the beginning of the L4 stage for precocious alae formation.
h
P > 0.05 compared to RG733 treated with lin-28 RNAi at the corresponding stage.
i
P < 0.001 compared to RG733 treated with hbl-1 RNAi at the corresponding stage.
j
P < 0.001 compared to ME357-untreated L4 animals.
k
P > 0.05 compared to RG733 treated with hbl-1 RNAi at the corresponding stage.