Table 3. Cytoplasmic incompatibility assays for D. teissieri lines.
| Cross | Infection Status | Mean Hatch Rate (± SD) | N | P Value | |
|---|---|---|---|---|---|
| Female | Male | ||||
| 1. L18(I) × L3(U) | U | I | 0.61 ± 0.40 | 29 | 0.028 |
| I | U | 0.84 ± 0.22 | 28 | ||
| 2. L11(I) × L3(U) | U | I | 0.84 ± 0.08 | 17 | <0.0001 |
| I | U | 0.96 ± 0.06 | 17 | ||
| 3. L13(I) × L3(U) | U | I | 0.79 ± 0.26 | 20 | 0.071 |
| I | U | 0.88 ± 0.13 | 18 | ||
| 4. F1(L18 × L3) × L3 | U | I | 0.86 ± 0.18 | 17 | 0.260 |
| I | U | 0.92 ± 0.08 | 15 | ||
| 5. F1(L18 × L3) × L4 | U | I | 0.81 ± 0.20 | 20 | <0.0001 |
| I | U | 0.97 ± 0.02 | 19 | ||
| 6. F1(L11 × L18) × L3 | U | I | 0.84 ± 0.31 | 18 | 0.12 |
| I | U | 0.89 ± 0.24 | 18 | ||
| 7. F1(L13 × L18) × L3 | U | I | 0.93 ± 0.07 | 14 | 0.002a |
| I | U | 0.91 ± 0.25 | 14 | ||
| 8. L18L11 × L3(U) | U | I | 0.83 ± 0.19 | 18 | 0.002b |
| I | U | 0.85 ± 0.29 | 23 | ||
| 9. L18L13 × L3(U) | U | I | 0.71 ± 0.34 | 20 | 0.003 |
| I | U | 0.92 ± 0.08 | 17 | ||
| 10. L5L18(I) × L3(U) | U | I | 0.66 ± 0.35 | 19 | 0.105 |
| I | U | 0.79 ± 0.28 | 19 | ||
| 11. L4L18(I) × L3(U) | U | I | 0.69 ± 0.35 | 16 | 0.279 |
| I | U | 0.73 ± 0.32 | 16 | ||
| 12. L18 × L13 | I (L13) | I (L18) | 0.86 ± 0.30 | 19 | 0.556 |
| I (L18) | I (L13) | 0.80 ± 0.33 | 24 | ||
| 13. L18 × L11 | I (L11) | I (L18) | 0.76 ± 0.40 | 27 | 0.820 |
| I (L18) | I (L11) | 0.89 ± 0.23 | 20 | ||
Data are mean hatch rates of eggs laid over 5 days. For backcross experiments, superscripts denote source isofemale line for the Wolbachia. For crosses that used F1, parental lines are listed in parentheses, female first. P values are for one-tailed Wilcoxon tests, with the exception of II crosses (12 and 13), which are two tailed. Statistically significant differences between reciprocal crosses at P < 0.05 are highlighted in bold.
The one-sided MWU indicates that UI tend to produce lower egg hatch than do IU crosses despite UI having lower mean egg hatch than IU. This result is driven by the larger variance in egg hatch for UI (0.25 SD) vs. IU (0.07 SD)—one IU replicate produced 0% egg hatch (0/27). The lower median egg hatch of UI (0.94) vs. IU (0.99) supports this conclusion (Figure 4).
This difference is highly statistically significant despite similar means as indicated by the lower median egg hatch rate of UI (0.89) vs. IU (0.98) crosses. Two replicates within the IU class produced 0% eggs hatch (0/34 and 0/36) (Figure 4).