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. 2004 Oct;42(10):4581–4585. doi: 10.1128/JCM.42.10.4581-4585.2004

TABLE 1.

Comparison of medium formulations for BacT/ALERT FAN, FN, and SN anaerobic media

Feature Anaerobic FAN FN SN
Volume (mL) 40 40 40
Tryptic soy broth (%, wt/vol) 2.0
Pancreatic digest of casein (%, wt/vol) 1.70
Brain heart infusion solids (%, wt/vol) 1.5 0.1
Papaic digest of soybean meal (%, wt/vol) 0.3
Sodium polyanetholesulfonate (%, wt/vol) 0.05 0.044 0.035
Pyridoxine HCl (%, wt/vol) 0.001 0.001
Menadione (%, wt/vol) 0.00005 0.00005 0.00005
Hemin (%, wt/vol) 0.0005 0.0005 0.0005
Activated charcoal (%, wt/vol) 8.5
Ecosorb (%, wt/vol) 7.0
l-Cysteine and other complex amino acid and carbohydrate substrates in purified water As needed As needed As needed
Atmosphere Carbon dioxide and nitrogen under vacuum Nitrogen under vacuum Carbon dioxide and nitrogen under vacuum
Other proprietary adjustments As needed As needed As needed