Volume (mL) |
40 |
40 |
40 |
Tryptic soy broth (%, wt/vol) |
|
2.0 |
|
Pancreatic digest of casein (%, wt/vol) |
|
|
1.70 |
Brain heart infusion solids (%, wt/vol) |
1.5 |
0.1 |
|
Papaic digest of soybean meal (%, wt/vol) |
|
|
0.3 |
Sodium polyanetholesulfonate (%, wt/vol) |
0.05 |
0.044 |
0.035 |
Pyridoxine HCl (%, wt/vol) |
0.001 |
0.001 |
|
Menadione (%, wt/vol) |
0.00005 |
0.00005 |
0.00005 |
Hemin (%, wt/vol) |
0.0005 |
0.0005 |
0.0005 |
Activated charcoal (%, wt/vol) |
|
8.5 |
|
Ecosorb (%, wt/vol) |
7.0 |
|
|
l-Cysteine and other complex amino acid and carbohydrate substrates in purified water |
As needed |
As needed |
As needed |
Atmosphere |
Carbon dioxide and nitrogen under vacuum |
Nitrogen under vacuum |
Carbon dioxide and nitrogen under vacuum |
Other proprietary adjustments |
As needed |
As needed |
As needed |