TABLE 1.
Comparison of medium formulations for BacT/ALERT FAN, FN, and SN anaerobic media
Feature | Anaerobic FAN | FN | SN |
---|---|---|---|
Volume (mL) | 40 | 40 | 40 |
Tryptic soy broth (%, wt/vol) | 2.0 | ||
Pancreatic digest of casein (%, wt/vol) | 1.70 | ||
Brain heart infusion solids (%, wt/vol) | 1.5 | 0.1 | |
Papaic digest of soybean meal (%, wt/vol) | 0.3 | ||
Sodium polyanetholesulfonate (%, wt/vol) | 0.05 | 0.044 | 0.035 |
Pyridoxine HCl (%, wt/vol) | 0.001 | 0.001 | |
Menadione (%, wt/vol) | 0.00005 | 0.00005 | 0.00005 |
Hemin (%, wt/vol) | 0.0005 | 0.0005 | 0.0005 |
Activated charcoal (%, wt/vol) | 8.5 | ||
Ecosorb (%, wt/vol) | 7.0 | ||
l-Cysteine and other complex amino acid and carbohydrate substrates in purified water | As needed | As needed | As needed |
Atmosphere | Carbon dioxide and nitrogen under vacuum | Nitrogen under vacuum | Carbon dioxide and nitrogen under vacuum |
Other proprietary adjustments | As needed | As needed | As needed |