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. 2017 Jan 10;7:2020. doi: 10.3389/fpls.2016.02020

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Copyright © 2017 Bromilow, Gethings, Langridge, Shewry, Buckley, Bromley and Mills.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of extraction buffer on gluten protein identifications. (A) Summary of the total number of proteins identified for all three extractions that are common and unique to the two mass spectrometry platforms when a criteria of three unique peptides is set. (B) Effect of extraction conditions platform on protein identification on QTOF when a criteria of three unique peptides is set. (C) Quantification curve of all proteins identified across the three extractions with E1, E2, and E3 being shown in red, green and blue respectively. Data are summarized in Data Sheet 1.