Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jan 2;216(1):83–92. doi: 10.1083/jcb.201607066

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Schendzielorz et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Protein import is impaired in Tim50-depleted mitochondria. (A) Steady-state Western blot analysis of WT and Tim50-depleted mitochondria. (B) Δψ of isolated mitochondria was assessed using the Δψ-sensitive dye DiSC₃(5). Fluorescence was recorded before and after addition of valinomycin. (C–F) ³⁵S-labeled precursors were imported into isolated mitochondria, and import stopped at the indicated time points with antimycin A, valinomycin, and oligomycin (AVO). Samples were PK treated and analyzed by SDS-PAGE and autoradiography. Results are presented as mean ± SEM. n = 3. The longest import time of the WT sample was set to 100%. m, mature protein.