Figure 2.

pam17Δ mitochondria display similar import defects as mitochondria lacking Tim50. (A) WT and Tim50-depleted mitochondria were solubilized with digitonin and subjected to α-Tim23 immunoisolation. Samples were analyzed by Western blotting. Total, 10%; elution, 100%. (B) Quantification of mean red/green fluorescence intensities from WT and pam17Δ cells. For each condition, three independent clones were analyzed and 150–1,500 cells were quantified. Results are presented as mean ± SEM. n = 3. (C) Δψ of WT and pam17Δ mitochondria was assessed as described in Fig. 1 B. (D–G) ³⁵S-labeled precursors were imported as described in Fig. 1 (C–F). p, precursor; m, mature protein. (H) Comparison of import efficiency of indicated ³⁵S-labeled precursors into Tim50-depleted or pam17Δ mitochondria after 15 min (results from Fig. 1 [C–F] and D–G).