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. 2017 Jan 2;216(1):83–92. doi: 10.1083/jcb.201607066

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© 2017 Schendzielorz et al.

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Figure 3. — Pam17 plays a motor-independent role in protein import. (A and B) The inward driving force of the motor was assessed using ³⁵S-labeled b₂(167)_Δ-DHFR (A and B) or b₂(220)-DHFR (B alone) in the presence of MTX. After an initial import reaction, membrane potential was dissipated with valinomycin. The precursor was chased in a second incubation step for indicated time points before PK was added. The amount of processed intermediate was quantified (100%: amount of processed intermediate without protease treatment). Results are presented as mean ± SEM. n = 3. (C) Schematic representation of F₁α, F₁β, pF₁α-F₁β, and pF₁β-F₁α. For pF₁α-F₁β, the first 38 aa of F₁α were fused to the mature part of F₁β (40–end). For pF₁β-F₁α, the first 45 aa of F₁β were fused to the mature part of F₁α (36–end). (D and E) ³⁵S-labeled pF₁α-F₁β and pF₁β-F₁α were imported into isolated mitochondria from indicated strains as described in Fig. 1. p, precursor; m, mature protein.