Fig. 4.

Functional characterization of GhLMI1-D1b using VIGS. (A) Representative sixth true leaf and 4-wk-old plants from VIGS experiment showing the reversion to normal leaf shape in the GhLMI1-D1b silencing treatment. (B) Relative transcript levels of candidate genes in the GhLMI1-D1b silenced and control LA213-Okra plants (n = 3) confirmed the effective knockdown of GhLMI1-D1b. Asterisks represent statistically significant differences as determined by unpaired t tests at *P < 0.05. (C) Transcript levels of GhLMI1-D1a were unaffected by VIGS treatment, confirming silencing was specific to GhLMI1-D1b.