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No significant changes in the activation of ERK, JNK, or p38-MAPK were detected by Western blot after pFUS+MB. Phosphorylation of ERK1/2, 44/42 MAPK, JNK, and p38-MAPK did not increase significantly after pFUS+MB compared with the total proteins, indicating that these pathways were not activated. All experiments and analysis were performed as described in Fig. 4.
