Fig. S1.

EET biosynthesis and hydrolysis. (A) Cellular cytochrome P450 enzymes can catalyze the epoxidation of arachidonic acid, a 20-carbon, ω-6, polyunsaturated fatty acid, resulting in the biosynthesis of four EET regioisomers. (B) The 14,15-EET is hydrolyzed by Cif-WT but not by catalytic mutant Cif-D129S. A total of 1 µM 14,15-EET was incubated with 1 µM recombinant Cif-WT or Cif-D129S (a catalytically inactive mutant) for 45 min. The reactions were stopped with ice-cold MeOH, extracted, and analyzed by ELISA for 14,15-EET (gray bars) and 14,15-DHET (black bars) concentrations. ***P < 0.001, ****P < 0.0001; one-way ANOVA Tukey’s post hoc test; n ≥ 3; mean ± SD.