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Analysis of SC density in the intrapancreatic nerves of PDAC patients with or without pain. PDAC patients were divided into two groups depending on their preoperative pain status (no pain vs. pain; n = 10 patients each). The paraffin-embedded histological specimens were double-immunostained against the SC markers S100 and GFAP, and the number of DAPI+ nuclei in each nerve was counted manually. (Scale bars: 40 µm.)
