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. 2016 Dec 20;114(1):172–177. doi: 10.1073/pnas.1616459114

Fig. 1.

Fig. 1.

Gene expression and phenotype analysis of BBX32. Relative gene expression analysis of BBX32 under diurnal conditions (12L:12D) (A) and free running conditions (LL) (B) at 22 °C. Wild-type (Col-0) seedlings were grown in 12L:12D for 7 d (A) and 12L:12D for 6 d and transferred to continuous light (LL) and harvested 24–68 h later (B). Error bars represent the SEM of biological triplicates. Experiments were independently repeated three times, each time with two biological replicates per genotype. (C) BBX32-OX:LHY-LUC+ activity under LL. Six-day-old seedlings entrained in 12L:12D were monitored for 5–7 d under LL. Values are shown as mean ± SEM; n = 20. (D) Relative amplitude error of WT and BBX32-OX seedlings imaged for 5 d under LL conditions calculated by using FFT-NLLS; n = 20. (E) Relative mRNA expression of LHY in WT and BBX32-OX. Six-day-old WT and BBX32-OX seedlings were grown in 12L:12D and transferred to continuous light (LL) and harvested 24–68 h later. (F) BBX32-AMI:LHY-LUC+ activity under LL. Six-day-old seedlings entrained in 12L:12D were monitored for 5–7 d under LL. Values are shown as mean ± SEM; n = 20. Experiments were repeated three times independently.