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. 2016 Dec 20;114(1):172–177. doi: 10.1073/pnas.1616459114

Fig. S1.

Fig. S1.

Gene expression of BBX32 and phenotypes. (A) Relative gene expression of BBX32 in clock mutants. Wild-type (Col-0) and clock mutant (cca1-1, toc1-4, elf3-3) seedlings were grown for 6 d in 12L:12D and transferred to continuous light (LL) on day 7 and harvested 24–60 h later. Error bars represent the SEM of biological triplicates. Experiments were repeated three times independently. Unshaded and shaded areas represent subjective light and dark periods, respectively. (B) Expression of BBX32 in mutants generated for this study. Wild-type (Col-0 and WS), Flag-tagged BBX32-OX, Flag-tagged BBX32 OX_col3, and BBX32 AMI_col3 seedlings were grown for 6-d in 12L:12D and harvested on day 7 at Zeitgeber Time (ZT) 0. Error bars represent the SEM of biological triplicates. Experiments were repeated three times independently with two biological replicates per genotype. **P ≤ 0.001. (C) Expression analysis of major clock genes in Flag-tagged BBX32-OX. Experiments were performed on 7-d-old seedlings grown in 12L:12D cycles at 22 °C. Error bars represent the SEM of biological triplicates. Experiments were repeated three times independently with two biological replicates per genotype. *P ≤ 0.05. Unshaded and shaded area represents light and dark period, respectively.