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. 2016 Dec 20;114(1):172–177. doi: 10.1073/pnas.1616459114

Fig. S3.

Fig. S3.

Gene expression and flowering time analyses of COL3. (A) Relative gene expression analysis of COL3 under diurnal and free running conditions at 22 °C. Wild-type (Col-0) seedlings were grown in 12L:12D for 7 d for diurnal and 6 d in 12L:12D and transferred to continuous light (LL) and harvested 24–60 h later. Error bars represent the SEM of biological triplicates. Experiments were repeated three times independently with two biological replicates per genotype. Unshaded and shaded areas represent light and dark period (diurnal) and subjective light and dark period (circadian), respectively. (B) Relative expression of COL3 in GFP-tagged COL3-OX lines generated for this study. Wild-type (Col-0), GFP-tagged COL3-OX seedlings were grown for 6 d in 12L:12D at 22 °C and were harvested on day 7 at ZT0. Error bars represent the SEM of biological triplicates. Experiments were repeated three times independently with two biological replicates per genotype. **P ≤ 0.001. (C) GFP-tagged COL3-OX flowers late in long days (16L:8D) at 22 °C. Data show the average number of leaves at bolting. Error bars represent SE (n = 12) **P ≤ 0.001; unpaired t test.