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. 2016 Dec 20;114(1):172–177. doi: 10.1073/pnas.1616459114

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Fig. S4. — Subcellular localization of BBX32 and BBX32 and COL3 protein expression. (A) BBX32 is localized to nuclear speckles. Enlarged image of the nuclei shows the size and number of speckles. 35S::GFP was used as a control. BF, bright field. (B) Protein expression analysis of Flag-tagged BBX32-OX; Flag-tagged BBX32 OX_col3, and GFP-tagged COL3-OX lines. Ten-day-old seedlings grown in 12L:12D conditions were harvested at ZT1, and Western blot analysis was performed. Anti-FLAG and anti-GFP antibodies were used for BBX32-OX; BBX32 OX_col3, and COL3-OX, respectively.