qPCR |
|
|
|
BBX32 RT-F |
TGTGTCTCAAGCTCCGAGCTATCG |
— |
— |
BBX32 RT-R
|
TTTCCCTCCCTCGCGCTCTGTTTA |
— |
— |
BBX32-AMI-F |
GCGGATGGCATTTTTGTAA |
— |
— |
BBX32-AMI-R |
CGCCGCTAAGAACACTCTCT |
— |
— |
LHY-F |
ACCAACGAAACAGGTAAGTGGCG |
— |
— |
LHY-R |
GTGCACAGTAGTACCATCGTTACC |
— |
— |
CCA1 -F |
AATGTGACCGGTGGGTTCATGG |
— |
— |
CCA1-R |
AAGTGCATAGCACGCGCCTTAC |
— |
— |
TOC1 -F |
CTTCAATCTACTTTTCTTCGGTGCT |
— |
— |
TOC1-R |
AATAGTAATCCAGCGCAATTTTCTTC |
— |
— |
CO-F |
AACGACATAGGTAGTGGAGAGAACAAC |
— |
— |
CO-R |
GCAGAATCTGCATGGCAATACA |
— |
— |
FT-F |
CAACCCTCACCTCCGAGAATAT |
— |
— |
FT-R |
TGCCAAAGGTTGTTCCAGTGT |
— |
— |
IPP2-F |
GTATGAGTTGCTTCTCCAGCAAAG |
— |
— |
IPP2-R |
GAGGATGGCTGCAACAAGTGT |
— |
— |
COL3-F |
TACCGTTGGTGCCTGAAAGTGG |
— |
— |
COL3-R |
ATAACTGCACAGCTGGCGTCTC |
— |
— |
Y1.5H |
|
|
|
FT.FR1.1.E-F |
CACCCTTATTTTTATTAATAATCTTAC |
−508 /−4 |
TOPO cloning primer |
FT.FR1.1-R |
CTTTGATCTTGAACAAACAGG |
|
FT.FR2.1.E-F |
CACCGAAGAGAAATAAAACAATTGATTTGG |
−975/−493 |
TOPO cloning primer |
FT.FR2.1-R |
TATTAATAAAAATAAGAAAAAAGTAGGG |
|
FT.FR3.1.E-F |
CACCCTGAGATGTTACAAA |
−1468/−956 |
TOPO cloning primer |
FT.FR3.1-R |
CAATTGTTTTATTTCTCTTCTTTTTTTA |
|
FT.FR4.1.E-F |
CACCCAAAAAGATACACTTTT |
−1960/−1461 |
TOPO cloning primer |
FT.FR4.1-R |
CATCTCAGTAATGTATTCATATTG |
|
FT.FR5.1.E-F |
CACCCGAACATTATGCGA |
−2451/−1948 |
TOPO cloning primer |
FT.FR5.1-R |
GTGTATCTTTTTGTTTTTTGATTTAAT |
|
FT.FR6.1.E-F |
CACCGTGATGCACTATATG |
−2571/−2366 |
TOPO cloning primer |
FT.FR6.1-R |
GTTTTGTCTTTATCAACTCCAC |
|
ChIP |
|
|
|
FT-Amp5-F |
CATGCGAAAATCTAGTGGAAGAAA |
−2537/−2514 |
— |
FT-Amp5-R |
AAGCCGTGATGTTCCTAAACAC |
−2335/−2314 |
— |
FT-Amp4-F |
GCGACTGCGACCTATTTTTTTC |
−1860/−1839 |
— |
FT-Amp4-R |
GCTATATGCACTTTTTAACGACTAGC |
−1692/−1667 |
— |
FT-Amp3-F |
CCTACAGTTGTTAGGCTATGGT |
−1354/−1333 |
— |
FT-Amp3-R |
CTAACCATCCATTTGCACGAC |
−1179/−1159 |
— |
FT-Amp2-F |
GACTATTCTCAAATGTCCTGGTTC |
−782/−759 |
— |
FT-Amp2-R |
GGTACCGCCAAGTTTATAGAGA |
−540/−519 |
— |
FT-Amp1-F |
GTGTATTAGTGTGGTGGGTT |
−297/−278 |
— |
FT-Amp1-R |
TGATCTTGAACAAACAGGTG |
−26/−7 |
— |
C1-F |
GAGACCCTCTTATAGTAAGCAGA |
— |
Locus related control |
C1-R |
GTATAGAAGTTCCTGAGGTCTTC |
— |
Locus related control |
C2-F |
TTCTTTTTTCATGAAGATGGACCC |
— |
Locus related control |
C2-R |
GAACATCAATTTTCTATATCTTTTTGCAG |
— |
Locus related control |
UBQ10-F/E-F |
TCCAGGACAAGGAGGTATTCCTCCG |
— |
Locus unrelated control |
UBQ10-R/E-R |
CCACCAAAGTTTTACATGAAACGAA |
— |
Locus unrelated control |
Cloning |
|
|
|
BBX32-F |
CACCTGTGTCTCAAGCTCCGAGCTATCG |
— |
TOPO cloning primer |
BBX32-R |
TAAACAGAGCGCGAGGGAGGGAAA |
— |
Full-length cDNA |
COL3-F |
CACCATGGCGTCGTCGTCAAGACTTTGCG |
— |
TOPO cloning primer |
COL3-R |
TCAGAAACTCGGAACAACACCG |
— |
Full-length cDNA |
Reverse COL3 N-term +Stop |
TCAACCGCCATCTTCATCTAC |
— |
— |
Reverse COL3 C-term |
TCAGAAACTCGGAACAACACCG |
— |
— |
BBX32 N term -F |
CACCATGGTGAGCTTTTGCGAG |
— |
TOPO cloning primer |