Figure 7.

Fbxo28 depletion leads to aberrant chromosome morphology. HeLa S/A shFbxo28 cells induced with doxycycline for 72 h were treated with Colchicine for 1 h, harvested, and Giemsa-stained chromosome spreads were prepared. (A) Representative images show normal chromosome spreads in control (-Dox) cells and aberrant chromosome spreads in Fbxo28-depleted cells (+Dox), Scale bar: 10 µm. (B) Quantification of the percentage of normal and aberrant chromosome spreads in control or Fbxo28-depleted cells from 3 independent experiments (n = 100 cells per experiment). Error bars in the graph represent SD. Student's t-test was used to calculate p-values. **denotes significance at P < 0.01.