Figure 6.

Persistence to phase-specific drugs is due to a Fucci-red subpopulation. Stationary cells are sorted by FACS according to the Fucci markers and viability marker. After exposure to chemotherapy agents, cells are washed and monitored by time-lapse microscopy to detect cell fate. (A) Scheme of the experimental layout. (B) Increased survival of the STP Fucci-red subpopulation compared to the Fucci-green subpopulation under the phase-specific drug AraC (one-tail Wilcoxon rank sum test p ≤ 0.05) (C) For the non-specific drug BCNU, no significant difference is observed (one-tail Wilcoxon rank sum test p > 0.4.) (B–C) Mean of 3 replicates presented. Error bars: SE.