Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Nov 29;45(1):15–25. doi: 10.1093/nar/gkw1005

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — Structure of sd-rxRNA, gene silencing efficacy, and cellular internalization. (A) Chemical modifications of sd-rxRNA include 2′-O-methyl, 2′-fluoro, phosphorothioate linkages, and cholesterol covalently linked at the 3′ end of the sense strand by a tetraethylene glycol (TEG) linker. (B) Dose response was performed in HeLa cells using PPIB-targeting sd-rxRNA (UNT: untreated cells). mRNA levels were measured by QuantiGene Assay (Affymetrix). (C) COS-7 cells were treated with 250 nM sd-rxRNA-DY547 (red) and visualized by total internal reflection (TIRF) microscopy (see Supplementary Video 1). Scale bar = 10 μm.