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. 2016 Nov 29;45(1):15–25. doi: 10.1093/nar/gkw1005

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — sd-rxRNA is internalized through a saturable pathway. (A) HeLa cells were treated with 0.5 μM sd-rxRNA-Cy3 and imaged by live confocal microscopy. Uptake was quantified by measuring total fluorescence intensity at specific time points. (B) HeLa cells were either untreated (control) or pre-incubated with 2 μM unlabeled sd-rxRNA for 10 or 60 min. Cells were then treated with 0.5 μM sd-rxRNA-Cy3 and imaged by live cell confocal microscopy. sd-rxRNA fluorescence intensity was measured in a region only 10 pixels from the nucleus. (C) Representative images used for quantification in panels B and D. Blue represents DAPI stain and red represents Cy3-labeled sd-rxRNA. Scale bar = 10 μm. (D) Corresponding plot profiles of lines drawn through images in panel C. Blue lines represent DAPI stain intensity and red lines represent Cy3-labeled sd-rxRNA intensity.