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sd-rxRNA preferentially associates with EGF compared to Tf by live cell TIRF. (A) COS-7 cells were simultaneously treated with 250 nM sd-rxRNA-Cy3, 2 ng/μl EGF-Alexa647, and 25 ng/μl Tf-Alexa488. After 10 min, the cells were washed with fresh media and imaged by TIRF. Representative time-lapse images are shown (see Supplementary Video 3). Scale bar = 10 μm. (B) Enlarged images from panel A. Scale bar = 2 μm.
