Figure 5.

Diversity context library to optimize Taxadiene Synthase activity. (A) Taxadiene synthase (TXS) catalyzes the cyclization of geranylgeranyl-pyrophosphate (GGPP) into taxadiene, an intermediate in a pathway to produce the cancer drug Paclitaxel. (B) C- and N-terminal GFP tags attached to TXS show punctate subcellular localization consistent with poor TXS solubility. (C) Gas chromatography-mass spectroscopy (GC-MS) analysis was used to quantify the production of taxadiene in strains expressing TXS in our diversity-context library. Shown is the fold-change in taxadiene titer over the production of the baseline strain (untagged P_TDH3–TXS). The bars represent the mean, and the error bars the standard deviation, for three to four biological replicates.