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. 2016 Nov 28;45(1):288–295. doi: 10.1093/nar/gkw1090

Figure 2.

Figure 2.

(A) Schematics of DNA constructs. (B) Schematic of L1Cy5–7OTD binding to GQ and structure of L1Cy5–7OTD. As the DNA construct does not have an acceptor, the FRET is at donor only level before binding of L1Cy5–7OTD. (C) A sample smFRET trace showing L1Cy5–7OTD binding and how dwell time is measured. (D) smFRET histogram for L1Cy5–7OTD binding to pd-3Ly1Lp12T. The blue lines are Gaussian fit peaks and the red line is the cumulative of two peaks. (E) Similar histogram and fits to pd-hGQ12T construct.