Table 1. Sequences of the DNA constructs used in this study.
Construct | Sequence (5′ to 3′) | pd-Comp |
---|---|---|
pd-3Ly1Lp12T | TGGCGACGGCAGCGAGGC TT GGGTTAGGGTTAGGGTTAGGG T12-Cy3 | Strand 1 |
pd-hGQ12T | Cy3-T12GGGTTAGGGTTAGGGTTAGGG TT TGGCGACGGCAGCGAGGC | Strand 2 |
pd-12T3Ly1Lp | Cy3-TT GGGTGGGTGGGTGGG T12TGGCGACGGCAGCGAGGC | Strand 2 |
pd-12ThGQ | Cy3-TT GGGTTAGGGTTAGGGTTAGGG T12TGGCGACGGCAGCGAGGC | Strand 2 |
pd-Cy312T3Ly1Lp | TT GGGTGGGTGGGTGGG T12TGGCGACGGCAGCGAGGC | Strand 3 |
Strand 1 | GCC TCG CTG CCG TCG CCA-Biotin | |
12ThGQ | T12GGGTTAGGGTTAGGGTTAGGG TT | |
12T3Ly1Lp | T12GGGTGGGTGGGTGGG TT | |
Strand 2 | Biotin- GCC TCG CTG CCG TCG CCA | |
Strand 3 | Biotin- GCC TCG CTG CCG TCG CCA-Cy3 |
The GQ forming sections are underlined. The parts forming the duplex stem are in bold. Strand 1 and Strand 2 are complementary to the bold sections of other strands. The column name ‘pd-Comp’ refers to partial duplex complement.