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. 2016 Nov 28;45(1):446–460. doi: 10.1093/nar/gkw1111

Figure 2.

Figure 2.

tmNusG and tmNusGΔ are autoinhibited. (A) 2D [1H,15N]-HSQC spectra of 150 μM 15N-tmNusG, black, and of 89 μM 15N-tmNusG in the presence of tmNusEΔ in a twofold molar excess, red. (B) 1D [1H,15N]-HSQC spectra of 278 μM 15N-tmNusG, black, and of 30 μM 15N-tmNusG in the presence of tmRho in equimolar concentrations, red. (C) 2D [1H,15N]-HSQC spectra of 100 μM 15N-tmNusGΔ, black, and of 74 μM 15N-tmNusGΔ in the presence of tmNusEΔ in a twofold molar excess, red. (D) 1D [1H,15N]-HSQC spectra of 348 μM 15N-tmNusGΔ, black, and of 27 μM 15N-tmNusGΔ in the presence of tmRho in equimolar concentrations, red. (E) 1D [1H,15N]-HSQC spectra of 348 μM 15N-tmNusGΔ, black, and of 25 μM 15N-tmNusGΔ in the presence of ecRNAP in equimolar concentrations, red.