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Binding of tmNusG-DII to ecRNAP and tmRNAP subunits. 1D [1H,15N]-HSQC spectra of 375 μM 15N-tmNusG-DII, black, and of 15N-tmNusG-DII in the presence, red, of (A) ecRNAP (25 μM each), (B) tmβ (30 μM each) and (C) tmβ’:tmω (30 μM each). Differences between the spectra of free 15N-tmNusG-DII in (A) and (B and C) are due to different temperatures in the experiments.
