Table 1. List of primer sequences and cloning strategies.

Constructs	Primer sequences	Template of the PCR	Cloning strategy
Construct 2	5′-tacagtgacgaccccaagtgtacatacacgcgccccaaatacat-3′	pT/neo	Ligate to SmaI site of pUC19
	5′-tacagtgacgaccccaagtgtacatacacgcgccttggagtcatta-3′
Construct 3	5′-gtacatacacgcgcttagtatttggtagcattgccttta-3′	pT/neo	Ligate the PCR products
	5′-gtacatacacgcgcttgactgtgcctttaaacagcttgg-3′
	5′-acttggggtcgtcaccaattgtgatacagtgaattataagtg-3′	pT/neo
	5′-acttggggtcgtcaccgaatgtgatgaaagaaataaaagc-3′
Construct 4	5′-gtacatacacgcgcttagtatttggtagcattgccttta-3′	pT/neo	Ligate the PCR products
	5′-gtacatacacgcgcttgactgtgcctttaaacagcttgg-3′
	5′-acttggggtcgtcaccaattgtgatacagtgaattataagtg-3′	Construct2
	5′-acttggggtcgtcaccgaatgtgatgaaagaaataaaagc-3′
Construct 5	5′-acttccgacttcaactgtaggggatcctctagagtcgacctg-3′	pT/neo	Ligate the PCR products
	5′-acttccgacttcaactgtagggtaccgagctcgaattcactg-3′
	5′-gtacatacacgcgccccaaatacatttaaactcactttttc-3′	pT/neo
	5′-gtacatacacgcgccttggagtcattaaaactcgtttttc-3′
Construct 6 (pT4)	5′-acttctgacccactgggaatgtgatgaaagaaataaaagc-3′	pT/neo	Ligate the PCR products
	5′-acttctgacccactggaattgtgatacagtgaattataagtg-3′
	5′-gtacatacacgcgcttagtatttggtagcattgccttta-3′	pT/neo
	5′-gtacatacacgcgcttgactgtgcctttaaacagcttgg-3′
Construct 7	5′-gtacatacacgcgcttagtatttggtagcattgccttta-3′	pT/neo	Ligate the PCR products
	5′-gtacatacacgcgcttgactgtgcctttaaacagcttgg-3′
	5′-acttctgacccactgggaatgtgatgaaagaaataaaagc-3′	Construct 5
	5′-acttctgacccactggaattgtgatacagtgaattataagtg-3′