Construct 2 |
5′-tacagtgacgaccccaagtgtacatacacgcgccccaaatacat-3′ |
pT/neo |
Ligate to SmaI site of pUC19 |
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5′-tacagtgacgaccccaagtgtacatacacgcgccttggagtcatta-3′ |
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Construct 3 |
5′-gtacatacacgcgcttagtatttggtagcattgccttta-3′ |
pT/neo |
Ligate the PCR products |
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5′-gtacatacacgcgcttgactgtgcctttaaacagcttgg-3′ |
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5′-acttggggtcgtcaccaattgtgatacagtgaattataagtg-3′ |
pT/neo |
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5′-acttggggtcgtcaccgaatgtgatgaaagaaataaaagc-3′ |
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Construct 4 |
5′-gtacatacacgcgcttagtatttggtagcattgccttta-3′ |
pT/neo |
Ligate the PCR products |
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5′-gtacatacacgcgcttgactgtgcctttaaacagcttgg-3′ |
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5′-acttggggtcgtcaccaattgtgatacagtgaattataagtg-3′ |
Construct2 |
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5′-acttggggtcgtcaccgaatgtgatgaaagaaataaaagc-3′ |
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Construct 5 |
5′-acttccgacttcaactgtaggggatcctctagagtcgacctg-3′ |
pT/neo |
Ligate the PCR products |
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5′-acttccgacttcaactgtagggtaccgagctcgaattcactg-3′ |
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5′-gtacatacacgcgccccaaatacatttaaactcactttttc-3′ |
pT/neo |
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5′-gtacatacacgcgccttggagtcattaaaactcgtttttc-3′ |
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Construct 6 (pT4) |
5′-acttctgacccactgggaatgtgatgaaagaaataaaagc-3′ |
pT/neo |
Ligate the PCR products |
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5′-acttctgacccactggaattgtgatacagtgaattataagtg-3′ |
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5′-gtacatacacgcgcttagtatttggtagcattgccttta-3′ |
pT/neo |
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5′-gtacatacacgcgcttgactgtgcctttaaacagcttgg-3′ |
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Construct 7 |
5′-gtacatacacgcgcttagtatttggtagcattgccttta-3′ |
pT/neo |
Ligate the PCR products |
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5′-gtacatacacgcgcttgactgtgcctttaaacagcttgg-3′ |
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5′-acttctgacccactgggaatgtgatgaaagaaataaaagc-3′ |
Construct 5 |
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5′-acttctgacccactggaattgtgatacagtgaattataagtg-3′ |
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