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. 2016 Aug 27;45(1):435–445. doi: 10.1093/nar/gkw745

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Regulation of endogenous cysteine hydropersulfide (CysSSH) by cystathionine beta synthase (CBS) and cystathionine gamma lyase (CTH). (A) Potential molecular origins of ms² modification of tRNA^Lys(UUU). (B) The efficiency of siRNAs against CBS (siCBS) and CTH (siCTH) in HeLa cells. n = 4, *P < 0.05, **P < 0.01. (C) The endogenous free cysteine levels in cells treated with control siRNA and siRNAs against CBS (siCBS) or CTH (siCTH) were determined by mass spectrometry and normalized to endogenous phenylalanine levels. n = 3 each. (D) The endogenous CysSSH levels in cells treated with control siRNA and siRNAs against CBS (siCBS) or CTH (siCTH) were visualized by the SSP4 probe. Bar = 0.1 mm. (E) Relative levels of CysSSH were estimated by quantification of the intensity of SSP4 in each sample. Results are expressed as the intensity relative to that in control cells. n = 13–20, ****P < 0.0001 versus control sample.