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. 2016 Aug 27;45(1):435–445. doi: 10.1093/nar/gkw745

Figure 2.

Figure 2.

(A) Regulation of ms2 modification by CysSSH in intact cells. Silencing of CBS and CTH significantly reduced the ms2 level of tRNALys(UUU). n = 4, ****P < 0.0001. (B) Cells treated with 500 μM BCA exhibited a significant decrease in endogenous CySSSH levels. n = 8–9, ****P < 0.0001. (C) Treatment with 500 μM BCA significantly decreased the ms2 modification levels of tRNALys(UUU). n = 4, ****P < 0.0001. (D) Inhibition of CBS and CTH with siCBS and BCA synergistically suppressed endogenous CysSSH levels. n = 4, *P < 0.05, **P < 0.01, ***P < 0.001. (E) Experimental design for rescuing ms2 modification by the exogenous CysSSH donor or H2S donor. (F) The CysSSH donor, but not (G) H2S donor, effectively reversed the suppression of ms2 modification levels of tRNALys(UUU) by silencing CBS or CTH. n = 4, ****P < 0.0001. n.s.: not significant.