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. 2016 Aug 27;45(1):435–445. doi: 10.1093/nar/gkw745

Figure 4.

Figure 4.

Detection of polysulfidation in CDKAL1. (A) Illustration of the polysulfide-specific biotin labeling assay used to identify the polysulfidation in proteins. (B) HeLa cells overexpressing Myc-CDKAL1 were subjected to a polysulfide-specific biotin labeling assay. Myc-CDKAL1 was detected in enriched proteins using streptavidin beads. (C) The polysulfidation levels in wild-type CDKAL1 (WT) or mutant CDKAL1 carrying Cys-to-Ala mutations in the UPF0004 domain (UPF 3CtoA), radical SAM domain (SAM 3CtoA) or UPF0004/radical SAM domains (UPF/SAM 6CtoA) were evaluated using a polysulfide-specific biotin labeling assay.