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. 2016 Sep 9;45(1):92–105. doi: 10.1093/nar/gkw814

Figure 1.

Figure 1.

SPOP regulates SETD2 stability in a proteasome-dependent manner. (A) HEK293 cells were transfected with HA-SETD2 or empty vector followed by DMSO or MG132 (10 μM) treatment. EGFP was co-transfected as control. HA immunoprecipitation and Western blot were carried out. (B) HEK293 cells were treated with DMSO or MG132 (10 μM) for 12 h. Endogenous SETD2 was immunoprecipitated and blotted with antibody against SETD2. β-actin served as control. (C) Schematic representation of SETD2 deletion mutants tagged with FLAG or HA. (D and E) Identification of the E3 ligase target region of SETD2. HEK293 cells were transfected with different Flag-tagged truncations of SETD2 for 48 h and then treated with or without MG132 (10 μM) for 12 h. Cell lysates were subjected to immunoblot (IB) with anti-FLAG antibody.