Figure 1.
(A) NGB mRNA level in three human cell lines quantified by qRT-PCR. The NGB level in SH-SY5Y cells was 300-fold of that in HeLa cells and 240-fold of that in K562 cells. ***P < 0.001 compared to HeLa (N = 3, mean ± SEM). (B and C) The 3C profiles in SH-SY5Y and HeLa cells of the 300 kb region surrounding the NGB gene. The anchor fragments are indicated by the dashed lines. Approximate positions and direction of transcription of genes, and EcoRI cutting sites within the area are displayed at the top. Significant differences between cell types were detected by Student's t test (*P < 0.05) (N = 3, mean ± SEM).