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. 2016 Oct 3;45(1):231–243. doi: 10.1093/nar/gkw887

Figure 2.

Figure 2.

AID-induced gene conversion. (A) An overview of the AID-induced DNA sequence changes responsible for Ig reversion. Ig light chain V gene diversity was analyzed in sorted GFP (+) IgLSce GFP cell line. Sequence changes, classified into gene conversion, non-homologous end joining (NHEJ), and somatic hypermutation, are summarized on the map. The events in the same horizontal lines were derived from the same plasmid clones. The regions corresponding to CDR1 are emphasized by frames. (B) Sequences around the I-SceI site with detailed gene conversion analysis. The number of analyzed sequences and its variation are shown. Dashes indicate nucleotides identical to the sequence of the reference sequence of the mother cell line, and brackets mark the borders of deletions. Gene conversion tracts are underlined and annotated with the name of the likely pseudo V (ψV) donors. (C) ψV usage and gene conversion tract length observed in AID-induced gene conversion. (D) An overview of Ig light chain gene sequences in AID−/− DT40. (E) Sequences around the I-SceI site from AID−/− DT40. (F) Pathway choice of Ig reversion induced by AID.