Figure 9.

The absence of physical interactions between Rad51 and E. coli DNA ligase. E. coli DNA ligase (1.5 μg) or Rad52 (1 μg) was mixed with Rad51 (1 μg) and incubated in an ice-water bath for 1 h. Then, anti-Rad51 antibody-protein A agarose was added to the mixture, and incubated in the ice-water bath for another 1 h, followed by centrifugation. The proteins bound to the anti-Rad51 antibody–protein A agarose (ppt) and the proteins left in the supernatants (sup) were analyzed by SDS-polyacrylamide electrophoresis. We repeated these experiments three times to confirm the reproducibility.