Fig. 6.

Altered ganglioside expression in adipose tissue from St3gal2-null mice. (A) Gangliosides from visceral adipose tissue from adult WT and St3gal2-null (null) mice (11 months old) were extracted, partially purified, subjected to silica gel TLC and stained with the sialic acid-specific resorcinol reagent. Samples were loaded at equivalent weights of adipose tissue. Ganglioside standards are identified at the left and adipose ganglioside assignments (based on migration and immuno-overlay) to the right. The image was uniformly contrast enhanced for presentation. (B) Images of stained TLC plates (not contrast enhanced) were subjected to quantitative image analysis using ImageJ software. Relative staining densities were adjusted for the number of sialic acids in each species and are expressed as percent of total gangliosides. This figure is available in black and white in print and in color at Glycobiology online.