Table II.
Phosphorylation of IR effector proteins IRS-1 and Akta in different tissues of 9-month-old WT and St3gal2-null mice
| Adipose | Liver | Skeletal muscle | ||||
|---|---|---|---|---|---|---|
| Genotype | IRS-P/IRS | Akt-P/Akt | IRS-P/IRS | Akt-P/Akt | IRS-P/IRS | Akt-P/Akt |
| WT | 2.3 ± 0.9 | 1.8 ± 0.5 | 1.0 ± 0.1 | 0.9 ± 0.1 | 1.1 ± 0.2 | 1.4 ± 0.1 |
| St3gal2-null | 0.7 ± 0.3 | 0.9 ± 0.1 | 0.7 ± 0.2 | 1.2 ± 0.1 | 1.2 ± 0.1 | 1.5 ± 0.1 |
| Null/WT ratio | 0.32 | 0.51 | 0.71 | 1.2 | 1.1 | 1.0 |
aPixel intensities of bands on images of immunoblots were analyzed using ImageJ and total density per band determined. Values for phosphoproteins (arbitrary units) were divided by values for total proteins of each type and are reported as ratios.