Fig. 5. Validation shows in vitro and in vivo effects for Hgs and Wwp1. In the competition assays, we measure the relative abundances of pre-B-cells with and without an shRNA against our gene of interest either in culture or transplanted into mice. We measure relative proportions at the time of morbidity using FACS. All plots are mean ± S.D. For all samples, n = 3, except for Hgs, and Wwp1 tissue samples where n = 4.