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. 2016 Oct 28;5(12):3512–3519. doi: 10.1002/cam4.900

Figure 2.

Figure 2

PVT1 regulated the expression of miR‐146a by inducing the methylation of CpG Island in its promoter. (A–B) miR‐146a expression level was down‐regulated with PVT1 overexpression and upregulated with PVT1 silencing. The expression level of miR‐146a was measured by qRT‐PCR in LNCaP, PC‐3, and DU145 cells transfected with either PCDNA3‐PVT1 or si‐PVT1. (C–D) DNMT1, DNMT3a, and DNMT3b expression levels were increased with PVT1 overexpression, and decreased with PVT1 silencing. The level of DNMT1, DNMT3a, and DNMT3b were analyzed using qRT‐PCR. (E–G) Methylation inhibitor 5‐azacytidine increased the miR‐146a expression. The expression level of miR‐146a was examined by qRT‐PCR in LNCaP, PC‐3, and DU145 cells. (H) PVT1 overexpression promoted the methylation of miR‐146a CpG islands. The methylation of miR‐146a CpG sites was analyzed by Methylation‐specific PCR (MSP) analysis in LNCaP, PC‐3, and DU145 cells. **P < 0.01