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. 2016 Oct 28;5(12):3512–3519. doi: 10.1002/cam4.900

Figure 3.

Figure 3

miR‐146a overexpression eliminated the effects of PVT1 knockdown on prostate cancer cells. (A–C) miR‐146a overexpression eliminated the effect of PVT1 knockdown on cell viability of prostate cancer cells. LNCaP, PC‐3, and DU145 cells were transfected with empty vector or miR‐146a target vector for 24 h and then infected with si‐PVT1. Relative cell numbers were evaluated using MTT analysis at the indicated time points. **P < 0.01 indicates victory+si‐Ctrl versus vector+si‐PVT1, # P < 0.05, ## P < 0.01 indicate vector+si‐Ctrl versus miR‐146a+si‐Ctrl. (D–F) miR‐146a overexpression blocked the effect of PVT1 knockdown on the apoptosis of prostate cancer cells. The percentage of apoptotic cells were analyzed with FACS. n.s., no significance; FACS, fluorescence activated cell sorter.