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. 2016 Nov 22;5(12):3564–3571. doi: 10.1002/cam4.960

Figure 2.

Figure 2

The expressions of CYP2C8 and P‐gp were upregulated after exposure to SR12813 in A549 cells. (A) The relative mRNA expression of pregnane X receptor (PXR) in A549 cells before and after treatment with SR12813, GAPDH as internal control. (B) Quantitation of PXR protein expression using immunofluorescent staining assay in A549 cells with SR12813 treatment, PXR was stained with yellow dye and cell nucleus was stained with blue dye. (C) The levels of mRNA expression of CYP2C8 and P‐gp after SR12813 treatment at different times. (D) Quantitation of CYP2C8 and P‐gp protein expression using immunofluorescent assay in A549 cells with SR12813 treatment, CYP2C8 was stained with red dye, P‐gp was stained with green dye, and cell nucleus was stained with blue dye. Data were presented as mean ± SD for three independent experiments. **P < 0.01 versus control group.