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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Methods Enzymol. 2016 Dec 7;584:229–253. doi: 10.1016/bs.mie.2016.10.025

Figure 5.

Figure 5

Kinetic analysis of membrane-immersed proteolysis. (A) Real-time reaction time courses showing an increase in reaction rate with increasing substrate concentration. Note that the two highest concentrations of substrate (black and red points/lines overlay, indicating enzyme saturation by substrate). (B) A plot of initial velocities against FITC-TatA substrate concentration (in mole percent) is modeled well by a Michaelis-Menten enzyme kinetics model, allowing KM and Vmax kinetic constants to be extracted for GlpG intramembrane proteolysis.