Table 1.

DETERGENT MICELLE SYSTEM PROPERTIES	IMPLICATIONS FOR KINETIC ANALYSIS
Sequestration: enzyme and substrate are in separate detergent micelles	Imicellar fusion/fission rates introduce additional and unaccounted kinetic step
Micelle Dilution Effect: concentration of micelles is the effective 'volume' of the reaction	kinetic parameters scale with both type and concentration of detergent, not just concentration of proteins
Cooperativity: detergents self-associate in a strongly cooperative manner	danger of misappropriating cooperativity to enzyme:substrate interaction
Solubility: limit of solubility imposed by maximal protein:detergent ratios	substrate solubility limit and/or distorted micelle structure may plateau reaction rates before enzyme is truly saturated
Altered Dynamics: increased protein dynamics in detergent	cleavage at ectopic sites in substrates or even non-substrates
Accessibility: altered accessibility of inhibitors to enzyme active- site	loverestimation of inhibitor efficacies
Altered Conformation: non-native environment changes enzyme conformation/function	catalysis may be enhanced, diminished, or even disallowed
Enzyme Bias: only some intramembrane proteases are active in detergent (many require a membrane environment)	kinetic properties of the subset of enzymes active in detergent may not be generally applicable to entire class of proteases