Figure 2. Nkx2.2^△endo mice recapitulate the Nkx2.2^KO phenotype.

(A–H) Ghrl⁺ cells are increased, Ins⁺ cells are greatly reduced, and Gcg⁺ cells are decreased at E15.5 and P0 in Nkx2.2^△endo mice (n = 3). (I–J) Gast⁺ cells are reduced in Nkx2.2^△endo embryos at E15.5 (n = 3). (K–L) SST⁺ cells are unchanged in Nkx2.2^△endo mice at P0 (n = 3). (M) Hormone gene expression changes recapitulate Nkx2.2^KO mice at E15.5 (Control n = 3, Nkx2.2^△endo n = 6) and E18.5 (Control n = 6, Nkx2.2^△endo n = 4). Ppy gene expression is increased at these time points. (N) Nkx2.2^△endo mice die neonatally. (O) qRT-PCR confirms increased Ppy at E15.5 in Nkx2.2^KO embryos (Control n = 3, Nkx2.2^KO n = 3). (*) p<0.05; (**) p<0.01; (***) p<0.001.

DOI: http://dx.doi.org/10.7554/eLife.20010.005

Figure 2—figure supplement 1. Nkx2.2^△endo mice display endocrine specification defects at P0.

(A–D) Low magnification images showing increased Ghrl⁺ cells, and decreased Ins⁺ cells and Gcg⁺ cells in Nkx2.2^△endo mice at P0 (n = 3). (E) Whole pancreas quantification of Gcg⁺, Sst⁺, and Ghrl⁺ cells normalized to pancreas area at P0. Nkx2.2^△endo mice display decreased Gcg⁺ cells, no change in Sst⁺ cells, and increased Ghrl⁺ cells (Control n = 3, Nkx2.2^△endo n = 5). (*) p<0.05; (**) p<0.01. A.U., arbitrary units.

DOI: http://dx.doi.org/10.7554/eLife.20010.006

Figure 2—figure supplement 2. PP cells are increased in Nkx2.2^△endo mice.

(A–D) Immunostaining for PP shows increased PP⁺ cells at E15.5 and P0 in Nkx2.2^△endo mice (n = 3). Arrow indicates a PP⁺Ins⁺ cell. (E–F) Low magnification images showing increased PP⁺ cells at P0 in Nkx2.2^△endo mice. (G) Whole pancreas quantification of Ins⁺ and PP⁺ cells normalized to the total number of Tom⁺ cells shows increased Ins^-PP⁺Tom⁺ cells and decreased Ins⁺PP^-Tom⁺ cells in Nkx2.2^△endo mice at P0 (n = 3). No changes in Ins⁺PP⁺Tom⁺ cells are detected. (**) p<0.01; (***) p<0.001.

DOI: http://dx.doi.org/10.7554/eLife.20010.007