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. Author manuscript; available in PMC: 2017 Jan 10.
Published in final edited form as: Dev Biol. 2016 Oct 4;420(1):136–147. doi: 10.1016/j.ydbio.2016.09.020

Fig. 5.

Fig. 5

Muscle- and epidermis-promoting transcription factors do not reprogram post-embryonic differentiated cells. (A–C) Expression of dpy-7::GFP reporter in epidermal cell nuclei in a C. elegans embryo (A, embryos are approximately 50 µM in length), L1 stage worm (B, approximately 250 µM in length DIC with GFP overlay), and head of adult worm (C, approximately 0.1 mm, DIC with GFP overlay). (D) Expression of dpy-7::GFP in a representative embryo after forced ubiquitous expression of the epidermal promoting GATA transcription factor ELT-1 (n=6). Expression of dpy-7::GFP 48 h after ectopic ELT-1 expression at the L1 (E) and adult (F) stage (n=24). (G-G′) Expression of myo-3::GFP reporter in the body wall muscle cells in a group of adult worms. (H–H′) Expression of myo-3::GFP reporter in a group of adult worms following ectopic HLH-1 expression. Widespread expression of myo-3::GFP is visible in retained embryos (yellow outline), but not adult worms.