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. Author manuscript; available in PMC: 2017 Jan 10.
Published in final edited form as: Dev Biol. 2016 Oct 4;420(1):136–147. doi: 10.1016/j.ydbio.2016.09.020

Fig. 7.

Fig. 7

Post-embryonic PHA-4 is not necessary for transdifferentiation. (A) Proximal gonad of gravid adult from control (empty vector, L4440) RNAi feeding. (B–C) Proximal gonad of adult after pha-4 RNAi feeding, (B) no embryos are visible, (C) many hatched larvae are present inside the worm, consistent with an egg laying defect. (D–E) IFB-2::CFP expression in the proximal gonad of worm from control (D) and pha-4 RNAi (E) feeding conditions 48 h after pulsed ELT-7 expression at the L3 stage. IFB-2::CFP is visible in the intestine “1” and proximal gonad “2” under both conditions. (F) Percentage of worms with ectopic IFB-2::CFP in both the pharynx and somatic gonad under the indicated experimental conditions. HS, heat shock to induce ELT-7. n, number of worms viewed.